module Bio.Base(
Nucleotide(..), Nucleotides(..),
Qual(..), toQual, fromQual, fromQualRaised, probToQual,
Prob'(..), Prob, toProb, fromProb, qualToProb, pow,
Word8,
nucA, nucC, nucG, nucT,
nucsA, nucsC, nucsG, nucsT, nucsN, gap,
toNucleotide, toNucleotides, nucToNucs,
showNucleotide, showNucleotides,
isGap,
isBase,
isProperBase,
properBases,
compl, compls,
Seqid,
Position(..),
shiftPosition,
p_is_reverse,
Range(..),
shiftRange,
reverseRange,
extendRange,
insideRange,
wrapRange,
w2c,
c2w,
findAuxFile
) where
import BasePrelude
#if MIN_VERSION_base(4,9,0)
hiding ( log1pexp, log1mexp )
#endif
import Bio.Util.Numeric ( log1pexp, log1mexp )
import Data.ByteString.Internal ( c2w, w2c )
import Data.Vector.Unboxed.Deriving ( derivingUnbox )
import System.Directory ( doesFileExist )
import System.FilePath ( (</>), isAbsolute, splitSearchPath )
import qualified Data.ByteString.Char8 as S
import qualified Data.Vector.Unboxed as U
newtype Nucleotide = N { unN :: Word8 } deriving ( Eq, Ord, Enum, Ix, Storable )
derivingUnbox "Nucleotide" [t| Nucleotide -> Word8 |] [| unN |] [| N |]
instance Bounded Nucleotide where
minBound = N 0
maxBound = N 3
newtype Nucleotides = Ns { unNs :: Word8 } deriving ( Eq, Ord, Enum, Ix, Storable )
derivingUnbox "Nucleotides" [t| Nucleotides -> Word8 |] [| unNs |] [| Ns |]
instance Bounded Nucleotides where
minBound = Ns 0
maxBound = Ns 15
nucToNucs :: Nucleotide -> Nucleotides
nucToNucs (N x) = Ns $ 1 `shiftL` fromIntegral (x .&. 3)
newtype Qual = Q { unQ :: Word8 } deriving ( Eq, Ord, Storable, Bounded )
derivingUnbox "Qual" [t| Qual -> Word8 |] [| unQ |] [| Q |]
instance Show Qual where
showsPrec p (Q q) = (:) 'q' . showsPrec p q
toQual :: (Floating a, RealFrac a) => a -> Qual
toQual a = Q $ round (10 * log a / log 10)
fromQual :: Qual -> Double
fromQual (Q q) = 10 ** ( fromIntegral q / 10)
fromQualRaised :: Double -> Qual -> Double
fromQualRaised k (Q q) = 10 ** ( k * fromIntegral q / 10)
newtype Prob' a = Pr { unPr :: a } deriving ( Eq, Ord, Storable )
type Prob = Prob' Double
derivingUnbox "Prob'" [t| forall a . U.Unbox a => Prob' a -> a |] [| unPr |] [| Pr |]
instance RealFloat a => Show (Prob' a) where
showsPrec _ (Pr p) = (:) 'q' . showFFloat (Just 1) q
where q = 10 * p / log 10
instance (Floating a, Ord a) => Num (Prob' a) where
fromInteger a = Pr (log (fromInteger a))
Pr x + Pr y = Pr $ if x >= y then x + log1pexp (yx) else y + log1pexp (xy)
Pr x Pr y = Pr $ if x >= y then x + log1mexp (yx) else error "no negative error probabilities"
Pr a * Pr b = Pr $ a + b
negate _ = Pr $ error "no negative error probabilities"
abs x = x
signum _ = Pr 0
instance (Floating a, Fractional a, Ord a) => Fractional (Prob' a) where
fromRational a = Pr (log (fromRational a))
Pr a / Pr b = Pr (a b)
recip (Pr a) = Pr (negate a)
infixr 8 `pow`
pow :: Num a => Prob' a -> a -> Prob' a
pow (Pr a) e = Pr $ a * e
toProb :: Floating a => a -> Prob' a
toProb p = Pr (log p)
fromProb :: Floating a => Prob' a -> a
fromProb (Pr q) = exp q
qualToProb :: Floating a => Qual -> Prob' a
qualToProb (Q q) = Pr ( log 10 * fromIntegral q / 10)
probToQual :: (Floating a, RealFrac a) => Prob' a -> Qual
probToQual (Pr p) = Q (round ( 10 * p / log 10))
nucA, nucC, nucG, nucT :: Nucleotide
nucA = N 0
nucC = N 1
nucG = N 2
nucT = N 3
gap, nucsA, nucsC, nucsG, nucsT, nucsN :: Nucleotides
gap = Ns 0
nucsA = Ns 1
nucsC = Ns 2
nucsG = Ns 4
nucsT = Ns 8
nucsN = Ns 15
type Seqid = S.ByteString
data Position = Pos {
p_seq :: !Seqid,
p_start :: !Int
} deriving (Show, Eq, Ord)
p_is_reverse :: Position -> Bool
p_is_reverse = (< 0) . p_start
data Range = Range {
r_pos :: !Position,
r_length :: !Int
} deriving (Show, Eq, Ord)
toNucleotide :: Char -> Nucleotide
toNucleotide c = if ord c < 128 then N (ar `U.unsafeIndex` ord c) else N 0
where
ar = U.replicate 128 0 U.//
( [ (ord x, n) | (x, N n) <- pairs ] ++
[ (ord (toUpper x), n) | (x, N n) <- pairs ] )
pairs = [ ('a', nucA), ('c', nucC), ('g', nucG), ('t', nucT) ]
toNucleotides :: Char -> Nucleotides
toNucleotides c = if ord c < 128 then Ns (ar `U.unsafeIndex` ord c) else nucsN
where
ar = U.replicate 128 (unNs nucsN) U.//
( [ (ord x, n) | (x, Ns n) <- pairs ] ++
[ (ord (toUpper x), n) | (x, Ns n) <- pairs ] )
Ns a `plus` Ns b = Ns (a .|. b)
pairs = [ ('a', nucsA), ('c', nucsC), ('g', nucsG), ('t', nucsT),
('u', nucsT), ('-', gap), ('.', gap),
('b', nucsC `plus` nucsG `plus` nucsT),
('d', nucsA `plus` nucsG `plus` nucsT),
('h', nucsA `plus` nucsC `plus` nucsT),
('v', nucsA `plus` nucsC `plus` nucsG),
('k', nucsG `plus` nucsT),
('m', nucsA `plus` nucsC),
('s', nucsC `plus` nucsG),
('w', nucsA `plus` nucsT),
('r', nucsA `plus` nucsG),
('y', nucsC `plus` nucsT) ]
isBase :: Nucleotides -> Bool
isBase (Ns n) = n /= 0
isProperBase :: Nucleotides -> Bool
isProperBase x = x == nucsA || x == nucsC || x == nucsG || x == nucsT
properBases :: [ Nucleotides ]
properBases = [ nucsA, nucsC, nucsG, nucsT ]
isGap :: Nucleotides -> Bool
isGap x = x == gap
showNucleotide :: Nucleotide -> Char
showNucleotide (N x) = S.index str $ fromIntegral $ x .&. 3
where str = S.pack "ACGT"
showNucleotides :: Nucleotides -> Char
showNucleotides (Ns x) = S.index str $ fromIntegral $ x .&. 15
where str = S.pack "-ACMGRSVTWYHKDBN"
instance Show Nucleotide where
show x = [ showNucleotide x ]
showList l = (map showNucleotide l ++)
instance Read Nucleotide where
readsPrec _ ('a':cs) = [(nucA, cs)]
readsPrec _ ('A':cs) = [(nucA, cs)]
readsPrec _ ('c':cs) = [(nucC, cs)]
readsPrec _ ('C':cs) = [(nucC, cs)]
readsPrec _ ('g':cs) = [(nucG, cs)]
readsPrec _ ('G':cs) = [(nucG, cs)]
readsPrec _ ('t':cs) = [(nucT, cs)]
readsPrec _ ('T':cs) = [(nucT, cs)]
readsPrec _ ('u':cs) = [(nucT, cs)]
readsPrec _ ('U':cs) = [(nucT, cs)]
readsPrec _ _ = [ ]
readList ('-':cs) = readList cs
readList (c:cs) | isSpace c = readList cs
| otherwise = case reads (c:cs) of
[] -> [ ([],c:cs) ]
xs -> [ (n:ns,r2) | (n,r1) <- xs, (ns,r2) <- readList r1 ]
readList [] = [([],[])]
instance Show Nucleotides where
show x = [ showNucleotides x ]
showList l = (map showNucleotides l ++)
instance Read Nucleotides where
readsPrec _ (c:cs) = [(toNucleotides c, cs)]
readsPrec _ [ ] = []
readList s = let (hd,tl) = span (\c -> isAlpha c || isSpace c || '-' == c) s
in [(map toNucleotides $ filter (not . isSpace) hd, tl)]
compl :: Nucleotide -> Nucleotide
compl (N n) = N $ n `xor` 3
compls :: Nucleotides -> Nucleotides
compls (Ns x) = Ns $ ar `U.unsafeIndex` fromIntegral (x .&. 15)
where
!ar = U.fromListN 16 [ 0, 8, 4, 12, 2, 10, 6, 14, 1, 9, 5, 13, 3, 11, 7, 15 ]
shiftPosition :: Int -> Position -> Position
shiftPosition a p = p { p_start = p_start p + a }
shiftRange :: Int -> Range -> Range
shiftRange a r = r { r_pos = shiftPosition a (r_pos r) }
reverseRange :: Range -> Range
reverseRange (Range (Pos sq pos) len) = Range (Pos sq (poslen)) len
extendRange :: Int -> Range -> Range
extendRange a r = r { r_length = r_length r + a }
insideRange :: Range -> Range -> Range
insideRange r1@(Range (Pos _ start1) length1) r2@(Range (Pos sq start2) length2)
| start2 < 0 = reverseRange (insideRange r1 (reverseRange r2))
| start1 <= length2 = Range (Pos sq (start2 + start1)) (min length1 (length2 start1))
| otherwise = Range (Pos sq (start2 + length2)) 0
wrapRange :: Int -> Range -> Range
wrapRange n (Range (Pos sq s) l) = Range (Pos sq (s `mod` n)) l
findAuxFile :: FilePath -> IO FilePath
findAuxFile fn | isAbsolute fn = return fn
| otherwise = go . maybe ["."] splitSearchPath . lookup "BIOHAZARD" =<< getEnvironment
where
go [ ] = return fn
go (p:ps) = doesFileExist (p </> fn) >>=
bool (return $ p </> fn) (go ps)